News
Screening for Multi-resistant E.coli (including E.coli O104:H4 strain)
AES CHEMUNEX presents an alternative protocol for ESBL-E. coli, including the O104:H4 strain, at the origin of the present outbreak in Germany.
This protocol is easy to carry out: one enrichment step in BPW at 37°C during 24h, followed by an isolation on the SEMIA chromogenic plate to incubate at 37°C during 24h.
Suspicious ESBL-E. coli form blue-green colonies on SEMIA.
The detection of the β-glucuronidase associated with the resistance to Ceftazidime give a high specificity to the medium.
The use of an antibiotic mix greatly enhances the inhibition of the background flora and makes the reading and interpretation easy.
