AES CHEMUNEX presents an alternative protocol for ESBL-E. coli, including the O104:H4 strain, at the origin of the present outbreak in Germany.

This protocol is easy to carry out: one enrichment step in BPW at 37°C during 24h, followed by an isolation on the SEMIA chromogenic plate to incubate at 37°C during 24h.

Suspicious ESBL-E. coli form blue-green colonies on SEMIA.  

The detection of the β-glucuronidase associated with the resistance to Ceftazidime give a high specificity to the medium.

The use of an antibiotic mix greatly enhances the inhibition of the background flora and makes the reading and interpretation easy.

Please note that AES CHEMUNEX also offers E. coli antisera (O104 and H4) for serotyping. (contact us for availability of this product in your country)